Workshop Details
Our mouse microsurgery and echocardiography workshop is designed for researchers to learn surgical techniques commonly used in cardiovascular and cerebrovascular research. Participants will learn survival and non-survival surgeries; also they will learn intravenous drug injection, blood collection, jugular vein catheterization (acute and chronic). They will learn intubation, anesthesia, surgical monitoring (including body temperature, EKG, heart rate) techniques which are commonly used during survival surgeries. All participants will have a chance to use real working pressure and pressure-volume catheters for measuring blood flow, blood pressure and left ventricular pressure-volume loops.
Using transthoracic echocardiography, participants will learn cardiac systolic and diastolic function measurement. Using pulsed wave Doppler echocardiography, participants will learn blood flow velocity measurement. Echo-guided pressure/volume catheter insertion into left ventricle will allow participants to record left ventricular pressure, pressure-volume loop, also record pressure-volume loop changes during stress echocardiography. By using contrast echocardiography they will learn myocardial blood flow measurement. Participants will also learn how to identify tumor size and tumor blood flow measurement by using contrast echocardiography
These are a hands-on sessions and participants will practice on mice. After survival surgery they will learn postsurgical care. The last day of the workshop, we will measure cardiac function after survival surgeries (myocardial infarction and transaortic banding) using echocardiography. For this workshop we are going to use Visual Sonic VEVO 2100 or VEVO 3100, VEVO-770 and Siemens Acuson Sequoia 512 ultrasound systems.
Daily Schedule
8-8:30 a.m.
Introductions
8:30-9:30 a.m.
- Acute and Chronic Surgery
- Anesthetics and Analgesics
- Post-surgical care and monitoring
9:30-10 a.m.
Coffee break
10 a.m. – Noon
Demonstration of the following:
- Dissection of vessels in mice(femoral artery and cartoid artery)
- Mouse jugular vein catheterization
- Pressure measurement (from femoral or cartoid arteries)
- Pressure volume measurement
Noon – 1:30 p.m.
Lunch
1:30- 5 p.m.
Practical session: Participants will select the type of vessel he or she would like to practice
Demonstration: Femoral artery blood flow and pressure measurement; computerized data collection. Participants who are interested can follow this demonstration.
5 – 5:30 p.m.
Questions and Answer Session
8-8:30 a.m.
Discussion of Previous Day
8:30-10 a.m.
Demonstration: Transaortic Banding and Transaortic De-banding
Mouse intubation
Practical Session: Participant’s choice
10 a.m. – Noon
Practical Session: Participant’s choice
Noon to 1:30 p.m.
Lunch
1:30 – 5 p.m.
Practical Session
5 – 5:30 p.m.
Questions and Answer Session
View the following videos to learn more about these areas: https://www.youtube.com/watch?v=Stnc6rilqYY or http://youtu.be/gLga0KHRE9k
8 – 8:30 a.m.
Discussion of Previous Day
8:30-10 a.m.
Demonstration: Myocardial Infarction (mouse)
ECG recording and analysis
Practical Session: Participant’s choice
10 a.m. – Noon
Practical Session: Myocardial Infarction (mouse)
Practical Session: Participant’s choice
Noon to 1:30 p.m.
Lunch (provided; companies to present their product)
1:30 – 5 p.m.
Practical: Participant’s choice
5:30 p.m.
Question and Answer Session
8 – 8:30 a.m.
Discussion of Previous Day
8:30- Noon
Transthoracic echocardiography
1. Evaluation of cardiac systolic and diastolic function, interpretation of echocardiographic data
2. Measurements of common, internal and external cartoid arteries diameter, wall motion and Doppler blood flow (mice).
Noon to 1 p.m.
Lunch
1 – 5 p.m.
Practical: Participant’s Choice
We will echo all mice survived after Transaortic constriction or after myocardial infarction. Participants will have a chance to echo their operated mice. After the workshop we will give all echo files to participants (per request).
5 – 5:30 p.m.
Question and Answer Session
8 – 8:30 a.m.
Discussion of Previous Day
8:30 a.m. – Noon
Demonstration:
Echo guided intra-myocardial and intra-ventricular injection
Evaluation of degree transaortic constriction
Stress echocardiography: measurement of cardiac function and blood pressure changes during stress test
Practical Sessions: Participant’s choice
Noon to 1 p.m.
Lunch
1 – 4 p.m.
Practical: Participant’s choice
4 p.m.
End of workshop
Certificate of completion of the workshop
Cutting the workshop cake
- Mouse coronary artery (LAD) isolation and cannulation
- Mouse femoral artery ligation (hindlimb ischemia).
- Contrast echocardiography (myocardial blood flow measurement)
- These surgeries are per request.
- $500 extra will be charged for each method.
Contact
Vahagn Ohanyan, M.D., Ph.D.
Assistant Professor
Phone: 330.325.6535
Email: vohanyan@neomed.edu