Prabodh Sadana, Ph.D.
Assistant Professor of Pharmaceutical Sciences
Dr. Sadana joined the Department of Pharmaceutical Sciences in November 2009. He received his Bachelors in Pharmacy, University of Delhi, New Delhi, India in 2000. In 2002, he received his Masters in Pharmacy (Pharmacology) from the University of Delhi, New Delhi, India. During his masters tenure, he was an instructor, Pharmacology course to the third year Bachelors of Pharmacy students at University of Delhi, New Delhi, India . In 2007, Dr. Sadana received his Ph.D. degree in Pharmacology, University of Tennessee, Memphis, TN.
He then worked for two years as a Post Doctoral Research Associate at St. Jude Children's Research Hospital, Memphis, TN.
2007: Ph.D. in Pharmacology, University of Tennessee, Memphis, TN
2002: Masters in Pharmacy (Pharmacology), University of Delhi, New Delhi, India
2000: Bachelors in Pharmacy, University of Delhi, New Delhi, India
2009-present: Assistant Professor, Department of Pharmaceutical Sciences, Northeastern Ohio Universities College of Pharmacy, Rootstown, OH
2007-2009: Post Doctoral Research Associate, St. Jude Children's Research Hospital, Memphis, TN
2000-2002: Instructor, Pharmacology, University of Delhi, New Delhi, India
Primarily my research interests lie in nuclear receptor (NR) physiology and pharmacology. Nuclear receptors are a big class of ligand activated transcription factors with 48 identified members in this class. These include proteins such as thyroid hormone receptor, glucocorticoid receptor, estrogen receptor, and androgen receptor as well as a number of orphan receptors with yet unidentified physiological ligands and function. All members of this superfamily share a modular structure and many share analogous molecular mechanism of action. Currently accepted model of NR action involves co regulator exchange at the C terminus of the DNA bound receptor. A great deal of specificity and complexity of NR actions is governed by differential coactivator recruitment at target gene promoters. The broad focus of my research is studying transcriptional responses to nuclear hormone receptors which are hormone activated nuclear receptors. Currently, the primary nuclear receptor of interest in my lab is the thyroid hormone receptor (THR).
Thyroid hormone is an essential endocrine hormone with broad effects in growth, development and metabolism. The genomic effects of T3 are regulated by thyroid hormone receptor (THR) bound to the TRE sequences in the promoters of its target genes. My lab is interested in delineating the molecular mechanisms of thyroid hormone action, its functional physiological consequences and developing means to modulate its activity and function. Among areas of active interest are:
- Molecular mechanisms that correlate thyroid hormone dysfunction with metabolic and cardiovascular effects.
- Regulatory mechanisms controlling expression of a and ß isoforms of the thyroid hormone receptor and means to modulate their activity.
- Role of specific coactivators in thyroid hormone action and means to modulate their activity and function.
Another intriguing aspect of NR biology pertains to the role of coactivators in NR function. Of particular interest to me is the PGC-1 coactivator family (PPARg coactivator). My lab is actively looking at underlying themes governing the role of PGC coactivators in normal physiology and disease.
We are carrying out integrated studies involving the use of various biochemical, cell biology, molecular biology and chemical biology approaches as well as animal models to address these issues.
P. Sadana, JY Hwang, RR Attia, LA Arnold, G Neale, RK Guy: Similarities and Differences between Two Modes of Antagonism of the Thyroid Hormone Receptor. ACS Chem Biol. 2011 Aug 15. [Epub ahead of print].
P. Sadana, H. Jong-Yeon, A.E. Arnold and R.K. Guy. Similarities and differences in the Effects of antagonism of ligand binding and antagonism of coregulatory protein binding to TR in T3 mediated gene expression. Undergoing revisions for ACS Chemical Biology.
P. Sadana, E.A. Park: PGC-1 isoforms stimulate transcription through their conserved N-terminus domain. Biochem J. May 1, 2007; 403(3):511-8
P. Sadana, Y. Zhang, S. Song, G.A. Cook, M.B. Elam, E.A. Park: Regulation of hepatic carnitine palmitoyltransferase I (CPT-I) gene expression by the peroxisomal proliferator activated receptor gamma coactivator-1beta (PGC-1ß). Mol Cell Endocrinol. Mar 15, 2007; 267(1-2):6-16.
Y. Zhang, K. Ma, P. Sadana, F. Chowdhury, S. Gaillard, D.P. McDonnell, M.B. Elam, E.A. Park Estrogen Related Receptors Stimulate Pyruvate Dehydrogenase Kinase Isoform 4 (PDK4) Gene Expression. J Biol Chem. Dec 29, 2006; 281(52):39897-906.
"Regulation of hepatic carnitine palmitoyltransferase I gene expression by the peroxisomal proliferator activated receptor gamma coactivator (PGC-1beta)." Presented in a session titled 'Genetic and Metabolic Approaches to Obesity' at the American Society of Biochemistry and Molecular Biology meeting, San Francisco, CA 2006.
"The role of PGC-1 isoforms in fatty acid and glucose metabolism." Presented in a session titled "Regulation of PPARs and PGC-1 in metabolism" at the American Society of Biochemistry and Molecular Biology meeting, San Diego, CA 2005.
P. Sadana. Thyroid hormone regulation of serum and glucocorticoid-inducible kinase 1 (SGK1). Cold Spring Harbor Meeting: Nuclear Receptor and Disease Aug-Sept 2010.
P. Sadana, J.Y. Hwang, A. Arnold and R.K. Guy. Selective Small Molecule inhibitors of thyroid hormone beta mediated gene expression based on inhibition of coactivator recruitment. Chemical approaches to nuclear receptors and metabolism. NIH workshop, April 2009.
J.Y. Hwang, P. Sadana, A. Arnold, F. Zhu, and R.K. Guy. Irreversible Thyroid Receptor Coactivator Modulators: structure-activity relationships (SARs) study and their application for chemical biology. The Pharmaceutical Society of Korea, 2009 Spring International Convention, Deajeon, Korea, April 2009.
P. Sadana, Y. Zhang, S. Song, G.A. Cook, M.B. Elam and E.A. Park. Regulation of hepatic carnitine palmitoyltransferase I gene expression by the peroxisomal proliferator activated receptor gamma coactivator (PGC-1beta). The Faseb Journal March 7 Vol 20(5), 2006.
F.A. Chowdhury, B. Baihav, P. Sadana, Y. Zhang and E.A. Park. Regulation of gluconeogenic gene expression by glucocorticoids, cAMP and cytokines. Experimental Biology meeting 2006. The Faseb Journal March March 7 Vol 20(5), 2006.
P. Sadana, Y. Zhang, Ke Ma, S. Song, and E.A. Park. Role of PGC-1 isoforms in fatty acid and glucose metabolism. The Faseb Journal March3, Vol 19(4), 2005.
"Pharmacokinetic/Pharmacodynamic Modeling: Concepts and Applications by Dr. Bill Jusko", May, 2006, Buffalo, NY.
American Association of Pharmaceutical Scientists–Southern Regional Discussion Group: PharmForum, May, 2004, University of Tennessee, Memphis, TN.