John Y.L. Chiang, Ph.D.

Professor of Biochemistry and Molecular Pathology
Department of Integrative Medical Sciences
College of Medicine

Education

Ph.D., State University of New York at Albany, N.Y. - 1976

B.S., National Taiwan Chung-Shing University, Taiwan - 1969

Professional Experience

Professor of Biochemistry and Molecular Pathology, Department of Integrative Medical Sciences, Northeast Ohio Medical University, Rootstown, Ohio - 1988-present 

Associate Professor of Biochemistry and Molecular Pathology, Department of Biochemistry, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio - 1983-1988  

Graduate Faculty, Cellular and Molecular Biology Program (Chair, 2000-present), Pharmacology Program, School of Biomedical Sciences, Kent State University - 1978-present 

Assistant Professor of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio - 1978-1983 

Postdoctoral Scholar, Supervisor (with Dr. M.J. Coon), Department of Biological Chemistry, The university of Michigan Medical School, Ann Arbor, Mich. - 1976-1978

Research Interest

Nuclear receptor regulation of bile acid metabolism in liver diseases, diabetes and obesity

Patents

Truncated human cholesterol 7α-hydroxylase, method of production and use thereof.     (U.S. Patent #5,420,028 issued on May 30, 1995, European Patents: No. 0648842, issued 2003). 

Cholesterol 7α-hydroxylase gene regulatory elements and methods of using them. (U.S. Patent #5,558,999 issued September 24, 1996).

Genomic DNA of human cholesterol 7α-hydroxylase, and methods of using it. (U.S. Patent #5,650,286 issued July 22, 1997). 

Transgenic mice expressing human cholesterol 7α-hydroxylase (U.S. Patent #5,663,483, issued September 2, 1997) 

Genomic DNA of human cholesterol 7α-hydroxylase, and methods of using it. (U.S. Patent #5,677,159 issued October 14, 1997).

Cholesterol 7α-hydroxylase gene regulatory elements and transcriptional factors. (U.S. Patent #5,753,431, issued May 19, 1998)  

Assay for agents that affect cholesterol 7α-hydroxylase expression and a characterization of its regulatory elements (US patent #5,821,057, issued October 13, 1998)

Genomic DNA of human cholesterol 7α-hydroxylase and method for using it. (US patent #5,851,780, issued December 22, 1998)

Recent Publications

Dr. Chiang's publications listed in PubMed

Li, T. and J. Y. L. Chiang. Bile acid signaling in liver metabolism and diseases. J. of Lipid, in press, 2011.

Chanda, D., Kim, D.K., Li, T., Kim, Y.H., Koo, S. H., Lee, C. H., Chiang, J., Choi, H. S. Cannabinoid receptor type 1 (CB1R) signaling regulates hepatic gluconeogenesis via induction of Endoplasmic Reticulum-bound transcription factor cAMP-responsive Element-binding Protein H (CREBH) in primary hepatocytes. J. Biol. Chem. 286: 27971-27979, 2011.

Li, T., Matozol, M., Boehme, S., Kong, B., Nilsson, L-M., Guo, G., and Chiang, J.Y.L. Overexpression of cholesterol 7α-hydroxylase promotes hepatic bile acid synthesis and secretion and maintains cholesterol homeostasis. Hepatology, 53:996-1006, 2011. 

Chiang, J.Y.L. Chapter 12: Bile acid metabolism in “Molecular Pathology of Liver Diseases”, S.P.S. Monga, ed, pp165-179, Springer, 2011.

Lee, J-M., Seo, W.-Y., Song, K-W., Chanda, D., Kim, Y. D., Kim, D.-K., Lee, M.-W., Ryu, D., Kim, Y.-H., Noh, J._R., Lee, C.-H., Chiang, J.Y.L., Koo, S.-H., Choi, H.-S. AMPK-dependent repression of hepatic gluconeogenesis via disruption of CREB-CRTC2 complex by orphan nuclear receptor small heterodimer partner. J. Biol. Chem. 285:32182-32191, 2010. 

Li, T., Owsley, E., Matozel, M., Hsu, P., Novak, C. M., and Chiang, J.Y.L. Transgenic expression of CYP7A1 in the liver prevents high fat diet-induced obesity and insulin resistance in mice. Hepatology 52:678-690, 2010. PMID: 20623580.

Song, K-H., Li, T., Owsley, E., and Chiang, J.Y.L. A putative role of microRNA in regulation of cholesterol 7α-hydroxylase expression in human hepatocytes. J. Lipid Res. 51:2223-2233, 2010. PMID: 20351063.

Han, S., Li, T., Ellis, E., Strom, S., and Chiang, J.Y.L. A novel bile acid-activated vitamin D receptor signaling in human hepatocytes. Mol. Endo. 24:1151-1164, 2010. PMID: 20371703.

Li, T., Chanda, D., Zhang, Y., Choi, H.-S. and Chiang, J. Y. L. Glucose stimulates cholesterol 7α-hydroxylase gene transcription in human hepatocytes. J. Lipid Res. 51:832-842, 2010.PMID: 19965590.

Chanda, D., Li, T., Song, K-H., Kim, Y-H., Sim, J., Lee, C. H., Chiang, J.Y.L., and Choi, H.-S. HGF family negatively regulates hepatic gluconeogenesis via induction of orphan nuclear receptor SHP in primary hepatocytes. J. Biol. Chem. 284: 28510-18521. 2009.

Li, T., Ma, H., Park, Y. J., Lee, Y-K., Strom, S. Moore, D.D. and Chiang, J. Y. L. Forkhead box transcription factor O1 inhibits cholesterol 7α-hydroxylase in human hepatocytes and in high fat diet-fed mice. Biochim Biophys Acta.1791:991-996, 2009. PMID: 19463968.

Chiang, J.Y.L. Bile acids: Regulation of Synthesis. J Lipid Res. 50:1955-1965, 2009.PMID: 19346330.

Chanda, D., Lee, C. H., Kim, Y-H., Noh, J.-R., Kim, D-K., Park, J-H., Hwang, J. H., Lee, M.-R., Jeong, K-H., Lee, I.-K., Yweon, G. R., Shong, M., Oh, G-T, Chiang, J. Y. L. and Choi, H.-S. Fenofibrate differentially regulates PAI-1 gene expressin via AMPK-dependent induction of orphan nuclear receptor SHP. Hepatology, 50:880-892, 2009.

Li, T. and Chiang, J.Y.L. Regulation of bile acid and cholesterol metabolism by PPARs. PPAR Research, 2009, 1-15, 2009.

Chiang, J. Y. L. Hepatocyte nuclear factor 4 regulation of bile acid and drug metabolism. Curr. Opion in Drug Metab. And Toxcol. 5:137-149, 2009.

Han, S. and Chiang, J.Y.L. Mechanism of Vitamin D receptor inhibition of cholesterol 7α-hydroxylase gene transcription in human heaptocytes. Drug Metab. Disp. 37:469-478. 2009.

Chiang, J. Y. L. Xenobiotic receptor cofactors and coregulators in “Nuclear receptors in Drug Metabolism”. Xie, Wen Ed. John Wiley &Sons, Inc. pp167-183, 2009.

Song, K.H., Li, T., Owsley, E., Strom, S. and J.Y.L. Chiang, J.Y.L. Bile acid activate fibroglast growth factor 19 signaling in human hepatocytes to inhibit cholesterol 7α-hydroxylase gene expression. Hepatology, 49:297-305. (2009).

T. Li, Ma, H and Chiang, J. Y. L.. TGF1, TNF, and insulin signaling crosstalk in regulation of the rat cholesterol 7α-hydroxylase gene expression. J. Lipid Res. 49:1981-1989, 2008.

Song, K-H, E, Ellis, S. Strom and Chiang, J. Y. L. Hepatocyte growth factor inhibits cholesterol 7α-hydroxylase expression in human hepatocytes. Hepatology, 46:1993-2002 (2007).

Shang, Q., Pan, L., Saumoy, M., Chiang, J. Y. L., Tint, G. S., Salen, G. and Xu, G. An overlapping binding site in the CYP7A1 promoter allows activation of FXR to override the stimulation by LXR. Am J. Physiol. G817-G823 (2007).

T. Li, and J.Y.L. Chiang. A novel role of transforming growth factors 1 in transcriptional repression of human cholesterol 7α-hydroxylase gene. Gastroenterology, 133:1660-1669 (2007)

Chiang, J. Y. L., T. Li, K. Song, M. Haghiac, and E. Owsley. Regulation of CYP7A1 by nuclear receptor signaling in human liver cells. In Falk Symposium 155: Bile acids: Biological Actions and Clinical Relevance. (D. Keppler, U. Beuers, U. Leuschner, A. Stiehl, M. Trauner, and G. Paumgartner, eds.), Springer, The Netherland, pp109-114  (2007).

Dikopoulos, N., Schmid, R. M., Bachem, M. Buttenschoen, K., Adler, G., Chiang, J.Y.L. and Weidenbach, H. Bile synthesis in rat models of inflammatory bowel diseases. Eur J Clin Invest. 37:222-230 (2007).

Li, T, W. Chen, and J. Y. L. Chiang. PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine. J. Lipid Res. 48:373-384 (2007).