Integrative Medical Sciences
Yeong-Renn Chen, Ph.D.
Department of Integrative Medical Sciences
College of Medicine
Ph.D., Biochemistry, Oklahoma State University, Stillwater, Okla. - 1994
B.S., Agricultural Chemistry, National Taiwan University, Taipei, Taiwan -1986
Associate Professor, Department of Integrative Medical Sciences, Northeast Ohio Medical University, Rootstown, Ohio - January 2010-present
Associate Professor, Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio - December 2007-December 2009
Assistant Professor, Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio - September 2002-December 2007
IRTA Fellow, NIEHS, National Institutes of Health, Research Triangle Park, N.C. - November 1997-August 2002
Research Associate, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Okla. - June 1994-November 1997
Graduate Research Assistant, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Okla. - August 1989-May 1994
Research Assistant, School of Pharmacy, National Taiwan University, Taipei, Taiwan - September 1988-July 1989
Compulsory Military Service (Army), Taiwan, Retired at the Rank of Staff Sargeant - September 1986-July 1988
Chen, C-A, Lin, C-H, Druhan, LJ, Wang, T-Y, Chen, Y-R, Zweier, JL (2011) Superoxide Induces eNOS Protein Thiyl Radical Formation: a novel mechanism regulating eNOS function and coupling. J. Biol. Chem. In press. PMID: 21666221.
Chen, J., Chen, C-L, Alevriadou, B.R., Zweier, JL, Chen, Y-R (2011) Excess Nitric Oxide Predisposes Mitochondrial Succinate-Cytochrome c Reductase to Produce Hydroxyl Radical. Biochim. Biophys. Acta (Bioenergetics). 1807, 491-502, PMID: 21406178.
Cai, M., Li, Y., Xu, Y., Swartz, H.M., Chen, C-L, Chen, Y-R, and He, G. (2011) Endothelial NOS Activity and Myocardial Oxygen Metabolism Define the Salvageable Ischemic Time Window for Ischemic Postconditioning. Am J Physiol Heart Circ Physiol. 300, H1069-77, PMID: 21217066.
Kang, T.P., Yun, J., Kaumaya, PT and Chen, Y-R (2011) Design and Use of Peptide-based Antibodies to Suppress Mitochondrial Electron Transfer Complexes. Biopolymer: Peptide Science. 96: 207-221, PMID: 20564035.
Chen, C-A, Wang T-Y, Varadharaj, S., Reyes, L, Hermann, C., Talukder, MAH, Chen, Y-R, Druhan, LJ, and Zweier, JL (2010) S-glutathionylation uncouples eNOS regulating its cellular and vascular function. Nature, 468: 1115-1118, PMID: 21179168
Chen, W., Druhan, L., Chen, C-A, Hemann, C., Chen, Y-R, Berka, V., Tsai, A-L, Zweier, J.L. (2010) Peroxynitrite-induced Destruction of the Tetrahydrobiopterin and Heme in Endothelial Nitric Oxide Synthase: Transition from Reversible to Irreversible Enzyme Inhibition. Biochemistry, 49: 3129-37
Yeh T. Steve, Angelos, MG, Chen, Y-R (2010) Simplified Method for Concentration of Mitochondrial Membrane Protein Complexes. Electrophoresis. 31, 1934-36.
Zhang, L., Chen, C-L, Kang, T.P., Hu, K, Green-Church, K, Chen, Y-R (2010) Peroxynitrite-Mediated Oxidative Modifications of Complex II: Relevance in the Myocardial Infarction. Biochemistry, 49, 2529-39.
Chen, J, Chen, C-L, Rawale, S, Chen, C-A, Zweier, JL, Kaumaya, PT, Chen, Y-R (2010) Peptide-based antibodies against glutathione-binding domains suppress Superoxide Production Mediated by Mitochondrial Complex I. J. Biol. Chem. 285 (5) 3168-3180.
Liu B, Zhu, X, Chen C-L, Hu K, Swartz HM, Chen Y-R, He G (2009) Opening the MitoKATP Channel and Decoupling of Mitochondrial Complex II and Complex III Contribute to Suppression of Myocardial Reperfusion Hyperoxygenation. Mol. and Cell. Biochem., 337, 25-38
Yeh T. Steve, Lee, H-L, Aune, SE, Chen, C-L, *Chen, Y-R, Angelos, MG (2009) Preservation of Mitochondrial Function with Cardiopulmonary Resuscitation in Prolonged Cardiac Arrest in Rats. J. Mol. Cell. Cardiology. 46, 789-97. *corresponding author
Zuo L, Chen Y-R, Reyes, L.A., Lee, H-L, Chen C-L, Villamena FA, Zweier JL (2009) The Radical Trap 5,5-Dimethyl-1-pyrroline N-oxide Exerts Dose-Dependent Protection Against Myocardial Ischemia-Reperfusion Injury Through Preservation of Mitochondrial Electron Transport. J. Pharmacology and Experimental Therapeutics. 329, 515-523.
Liu, B, Tewari, AK, Zhang, L, Green-Church, KB, Chen, Y-R, Zweier, JL, He, G (2009) Proteomic Analysis of Protein Tyrosine Nitration after Ischemic Reperfusion Injury: Mitochondria as the Major Target. Biochim. Biophys. Acta.- Protein and Proteomics. 389, 143-49.
Zou L, Chen Y-R, Chen C-L, Villamena FA, Zweier, JL (2008). The Radical Trap 5,5-Dimethyl-1-pyrroline N-oxide Exerts Dose-Dependent Protection Against Myocardial Ischemia-Reperfusion Injury Through Preservation of Mitochondrial Electron Transport. J. Pharmacology and Experimental Therapeutics. Revised and resubmitted.
Liu B, Tewari AK, Zhang L, Green-Church KB, Chen Y-R, Zweier JL, He G (2008). Proteomic Analysis of Protein Tyrosine Nitration after Ischemic Rerperfusion Injury: Mitochondria as the Major Target. Biochim. Biophys. Acta.-Protein and Proteomics. In press.
Zhang L, Xu H, Chen C-L, Green-Church KB, Freitas MA, Chen Y-R* (2008). Mass Spectrometry Profiles Superoxide-induced Formation of Mixed Intra-molecular Protein Disulfide on the FMN-binding Subunit of Mitochondrial Complex I. J. Amer. Soc. of mass Spectrom. 19 1875-86. *corresponding author
Chen C-L, Chen J., Rawale S, Varadharaj S, Kaumaya P, Zweier JL, Chen Y-R* (2008). Protein Tyrosine Nitration of Flavin Subunit is Associated with Oxidative Modification of Mitochondrial Complex II in the Post-ischemic Myocardium. J. Biol. Chem. 283 (41) 27991-28003. *corresponding author
Chen C-A, Druhan LJ, Varadharaj S, Chen Y-R, Zweier JL (2008). Phosphorylation of the Endothelial Nitric Oxide Synthase (eNOS) Increases Superoxide Generation from the Enzyme. J. Biol. Chem. 283 (41) 27038-47.
Chen Y-R* (2008). EPR Spin-trapping and Nano LC MS/MS Techniques for DEPMPO/*OOH and Immunospin-trapping with Anti-DMPO Antibody in Mitochondrial Electron Transport System. In Advanced Protocols for Oxidant Stress, Methods of Molecular Biology. Vol 477, 75-88. *corresponding author
Chen Y-R*, Chen C-L, Zweier JL (2007). Mitochondrial Complex II in the Post-ischemic Heart: Oxidative Injury and Protective Role of S-Glutathiolation. J. Biol. Chem. 282 (45) 32640-32654. *corresponding author
Zhu X, Liu B, Zhou S, Chen Y-R, Deng Y, Zweier JL, He G (2007). Ischemic Preconditioning Decreases Formation of Reactive Oxygen/Nitrogen Species and Regulates In Vivo Tissue Oxygenation and Oxygen Consumption in Postischemic Hearts. Am J Physiol Heart Circ Physiol. 293 (3): H1442-50