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Skeletal Biology Facilities

Facilities, Equipment, and Techniques

Imaging:

CT scan/bone densitometer

Imaging of intact bone structure

Laser scanning confocal microscope

Imaging of fluorescent, thick specimens

Green, red, far-red fluorescent tags

Laser capture microdissection microscope

Isolation of single cells/regions for further analysis

Epifluorescence/brightfield microscopes

Imaging of fluorescent or stained specimens

Green, red, blue fluorescent tags

Bio-Quant image analysis system

Quantitation of morphometric parameters

AMIRA Image Analysis

Three-dimensional reconstruction of cross-sectional images

Phase contrast inverted microscope

Imaging of live, unstained specimens

Scanning electron microscope

Imaging of surface topography

Transmission electron microscope

Imaging of thin-sectioned plastic embedded samples

3-D Laser Imaging

Quantification of micro-morphology

 

Molecular and protein analysis:

Quantitative real-time PCR

Analyze RNA levels in multiple samples

SDS-PAGE and Western blotting

Identify proteins by molecular weight and antibody binding

Agarose gel electrophoresis for DNA and RNA

Thermocyclers (PCR)

 

In vivo analysis:

Electromyography (EMG)

Strain gage

Sonomicrometry

High speed video and force platform data collection

Portable material properties testing equipment

 

Fossil analysis:

Fossil preparation equipment

Thin sectioning and polishing equipment for preparation of calcified tissues

 

Techniques:

Imaging modalities using all equipment listed

Quantitative real-time PCR

Microarray analyses

Western analyses

RNAi (using small, interfering RNAs to affect protein levels)

Cell culture

Transfection (introduction of DNA or RNA into cells)

Tissue engineering

Paraffin histology

Cryosectioning (LM and TEM levels)

Immunohistochemistry

Immunofluorescence

TUNEL analyses (apoptosis)

Collagen biochemistry

Fossil preparation

Thin sectioning and polishing of calcified tissues

Immunoelectron microscopy

Electromyography

Strain Studies

Sonomicrometry

Kinematic and Kinetic analyses